Research Description |
The underlying research theme in the Stern laboratory
is nuclear-cytoplasmic interactions. Within this framework, we study
how chloroplast genes and metabolic activities are regulated by the
products of nuclear genes, usually acting at the transcriptional or
post-transcriptional level. Chloroplasts, as the site of photosynthesis
as well as other metabolic pathways, have numerous roles in plant
development, and responses to environmental stimuli. Our laboratory
focuses on how these roles are modulated through intensive studies
of global and individual gene regulation under normal growth or stress
conditions. We use different techniques including genetics, plant
transformation, and biochemistry to unravel these processes. |
Publications |
Bohne, A. V., V. Ihrimovitch, A. Weihe, D. B. Stern.
2006. Chlamydomonas reinhardtii Encodes a Single sigma70-like
Factor Which Likely Functions in Chloroplast Transcription. Current
Genetics 49: 333-340.
Rymarquis, L. A., D. C. Higgs, D. B. Stern. 2006. Nuclear Suppressors
Define Three Factors that Participate
in Both 5’ and 3’ End
Processing of mRNAs in Chlamydomonas chloroplasts. The Plant
Journal 46: 448-461.
Cui, L., J. Leebens-Mack, L. S. Wang, J. Tang, L. Rymarquis, D.
B. Stern, C. W. dePamphilis. 2006. Adaptive Evolution of Chloroplast
Genome Structure Inferred Using a Parametric Bootstrap Approach. BMC
Evolutionary Biology 6: 13.
Irihimovitch, V., D. B. Stern. 2006. The
sulfur acclimation SAC3 kinase is required for chloroplast transcriptional
repression under sulfur limitation in Chlamydomonas reinhardtii.
Proc Natl Acad Sci USA 103: 7911-7916.
Bollenbach, T. J., H. Lange, R. Gutierrez, D. B. Stern, D. Gagliardi.
2005. RNR1,
a 3’-5’ Exoribonuclease Belonging to the RNR Superfamily,
Catalyzes 3’ Maturation of Chloroplast Ribosomal RNAs in Arabidopsis
thaliana. Nucleic Acids Research 33: 2751-2763.
Rymarquis, L. A., J. M. Handley, M. Thomas, D. B. Stern. 2005. Beyond
Complementation: Map-Based Cloning in Chlamydomonas reinhardtii.
Plant Physiology 137: 557-566.
Erickson, B., D. B. Stern, D. C. Higgs. 2005. Microarray
Analysis Confirms the Specificity of a Chlamydomonas reinhardtii Chloroplast
RNA Stability Mutant. Plant Physiology 137: 534-544.
Murakami, S., K. Kuehnle, D. B. Stern. 2005. A
Spontaneous tRNA Suppressor of a Mutation in the Chlamydomonas
reinhardtii Nuclear MCD1 Gene Required for Stability of the
Chloroplast petD mRNA. Nucleic Acids Research 33: 3372-3380.
Cohen, I., J. A. Knopf, V. Irihimovitch, M. Shapira. 2005. A
Proposed Mechanism for the Inhibitory Effects of Oxidative Stress
on Rubisco Assembly and Its Subunit Expression. Plant Physiology
137: 738-746.
Stern, D. B., M. R. Hanson, A. Barkan. 2004. Genetics
and Genomics of Chloroplast Biogenesis: Maize as a Model System.
Trends in Plant Science 9: 293-301.
Nishimura, Y., E. A. Kikis, S. L. Zimmer, Y. Komine, D. B. Stern.
2004. Antisense
Transcript and RNA Processing Alterations Suppress Instability of
Polyadenylated mRNA in Chlamydomonas chloroplasts. Plant
Cell 16: 2849-2869.
All publications |
