Ailong Ke
Assistant Professor

Ailong Ke

Phone

607-255-3945

Address

Department of Molecular Biology & Genetics
251 Biotechnology Building
Cornell University
Ithaca, NY 14853-2703

Email

Web Sites

Department Profile

Background

1995 B.S. in Biology. University of Science and Technology, China.
2002 Ph.D. in Biophysics. Johns Hopkins University School of Medicine. (Advisor: Dr. Cynthia Wolberger)
2002-05 Postdoc at University of California, Berkeley. (Advisor: Dr. Jennifer Doudna)

Research Description

Research in my lab centers around the biology of RNA. In eukaryotic cells, stable RNAs are transcribed with a 3’ extension that is subsequently trimmed from 3’-to-5’, whereas aberrantly processed RNAs are subject to rapid degradation. A conserved 300 – 400 kDa exoribonuclease protein complex, the exosome, plays crucial roles in both the RNA 3’-end formation and turnover processes (Fig. 1). The nuclear exosome is required for the 3’ end formation of 5.8S rRNA, small nuclear and nucleolar RNAs; and involved in the degradation of inefficiently spliced and hyper– or hypo-adenylated pre-mRNAs. The cytoplasmic exosome is required for the degradation of normal mRNAs as well as those containing premature termination codons, lacking termination codons, or bearing AU-rich elements near the 3’ untranslated region. Using X-ray crystallography and biochemical tools, we aim to understand the architecture, working mechanism, and the regulation of this multi-subunit machinery.

Publications

 

R.J. Spanggord, F. Siu, A. Ke, J.A. Doudna (2005), RNA-mediated interaction between the peptide binding and GTPase domains of the signal recognition particle. Nature Structural & Molecular Biology (accepted)

A. Ke and J.A. Doudna (2005), Catalytic strategies of self-cleaving ribozymes: Relics of an RNA world? The RNA World, 3rd Edition. (Book chapter, in print)

A. Ke and J.A. Doudna (2004), Crystallization of RNA and RNA-protein complexes. Methods 34, 408-414.

A. Ke, K. Zhou, F. Ding, J.H.D. Cate, and J.A. Doudna (2004), A conformational switch controls hepatitis delta virus ribozyme catalysis. Nature 429, 201-205