Richard A. Cerione
Professor of Pharmacology

Phone

607-253-3888

Address

Department of Molecular Medicine
C3-155 Veterinary Medical Center
College of Veterinary Medicine
Cornell University
Ithaca, New York 14853-2703

Email

Web Sites

Background

Ph.D. in Biochemistry, Rutgers University
Postdoctoral Fellow, Cornell University, Professor Gordon G. Hammes, Department of Chemistry, Characterization of the Nucleotide Sites on the Chloroplast H+-Adenosinetriphosphatase (Coupling Factor) Complex. Structural Mapping of Important Sites on the Reconstituted Coupling Factor Complex by Fluorescence Resonance Energy Transfer.
Senior Research Associate at Howard Hughes Medical Institute (Department of Medicine) Duke University Medical Center, Structure-Function Studies of Catecholamine (Adrenergic) Receptors and their Interactions with Adenylate Cyclase Utilizing Membrane Reconstitution and fusion Techniques.

National Institutes of Health Postdoctoral Fellow, Duke University
PEW Foundation Biomedical Scholar, Cornell University

Research Description

The research efforts of my laboratory have focused on understanding the molecular mechanisms by which signals are transmitted from cell surface receptors to biological effectors. In particular, we have been interested in identifying new signaling molecules that influence the growth and differentiation of mammalian cells. Three areas of research are currently being pursued in the laboratory.

Publications

Q. Feng, D. Baird, X. Peng, J. Wang, T. Ly, J.L. Guan, and R.A. Cerione. The Cool-1/alpha-Pix protein serves as an essential regulatory node for EGF receptor- and Src-mediated cell growth. Nature Cell Biology (in press).

N. Fidyk, J.B. Wang, and R.A. Cerione. Influencing cellular transformation by modulating the rates of GTP hydrolysis by Cdc42. Biochemistry 45: 7750-7762 (2006).

S. Majumdar, S. Ramachandran, and R.A. Cerione. New insights into the role of conserved, essential residues in the GTP-binding/GTP hydrolytic cycle of large G proteins. J. Biol. Chem. 281: 9219-9226 (2006).

Q. Lin, W. Yang, and R.A. Cerione. Measurement of epidermal growth factor receptor turnover and effects of Cdc42. In: Methods in Enzymology (W. Balch, C. Der and A. Hall, eds.), Elsevier Life Sciences. Vol. 406, 614-625 (2006).

J.J. Wakshlag, C.J. McNeill, M.A. Antonyak, C.E. Balkman, R. Fuji, R.A. Cerione, and R.L. Page. The prevalence and role of TGase up-regulation in canine and feline neoplasia. J. Comp. Path. 134: 202-210 (2006).

J.J. Wakshlag, M.A. Antonyak, J. Boehm, K. Boehm, and R.A. Cerione. Effects of tissue transglutaminase on beta-amyloid1-42-induced apoptosis. The Protein Journal 25: 83-94 (2006).

D. Baird, Q. Feng, and R.A. Cerione. Biochemical characterization of the Cool (Cloned-our-of library)/Pix (Pak-interactive exchange factor) proteins. In: Methods in Enzymology (W. Balch, C. Der and A. Hall, eds.), Elsevier Life Sciences. Vol. 406, 58-6 (2006).

W.J. Smith, B. Hamel, M.E. Yohe, J. Sondek, R.A. Cerione, and J.T. Snyder. A Cdc42 mutant specifically activated by intersectin. Biochemistry 44: 13282-13290 (2005).

R. Pereira and R.A. Cerione. A switch 3 point mutation in the ± subunit of transducin yields a unique dominant-negative inhibitor. J. Biol. Chem. 280: 35696-35703 (2005).

J.-B. Wang, W.J. Wu, and R.A. Cerione. Cdc42 and Ras cooperate to mediate cellular transformation by intersectin-L. J. Biol. Chem. 280: 22883-22891 (2005).

M. Osman and R. Cerione. Actin doesn't do the locomotion: Secretion drives cell polarization. In: Protein Trafficking: Mechanisms and Regulation (N. Segev, Editor) (2005).

D. Baird, Q. Feng, and R.A. Cerione. The Cool-2/alpha-Pix protein mediates a Cdc42-Rac signaling cascade. Current Biology 15: 1-10 (2005).

K.S. Brown, C.C. Hill, G.A. Calero, K.H. Lee, J.P. Sethna, and R.A. Cerione. The statistical mechanics of complex signaling networks: Nerve growth factor signaling. Phys. Biol. 1: 184-195 (2004).

M.A. Antonyak, A.M. Miller, J.M. Jansen, J.E. Boehm, C.E. Balkman, J.J. Wakshlag, R.L. Page, and R.A. Cerione. Augmentation of tissue transglutaminase expression and activation by epidermal growth factor inhibit doxorubicin-induced apoptosis in human breast cancer cells. J. Biol. Chem. 279: 41461-41467 (2004).

S. Majumdar, S. Ramachandran, and R.A. Cerione. Perturbing the linker regions of the alpha-subunit of transducin: A new class of constitutively active GTP-binding proteins. J. Biol. Chem. 279: 40137-40145 (2004).

Q. Feng, D. Baird, and R.A. Cerione. Novel regulatory mechanisms for the Dbl family guanine nucleotide exchange factor Cool-2/alpha-Pix. EMBO J. 23: 3492-3504 (2004).

W. Yang, J.M. Jansen, Q. Lin, S. Canova, R.A. Cerione, and C. Childress. Interaction of Cdc42-associated tyrosine kinase ACK2 with HSP90. Biochem. J. 382: 199-204 (2004).

G.R. Hoffman, and R.A. Cerione. Regulation of the Rho GTPases by RhoGDI. In: Rho GTPases. (Plenum Publishers, ed. Marc Symons) pp 32-43 (2004).

S. Ramachandran and R.A. Cerione. Stabilization of an intermediate activation state for transducin by a fluorescent GTP analogue. Biochemistry 43: 8778-8786 (2004).

J. Erickson and R.A. Cerione. Structural elements, mechanism and evolutionary convergence of Rho protein-guanine nucleotide exchange factor complexes. Biochemistry 43: 837-842 (2004).

L.A. Fortier, M.M. Deak, S.A. Semevolos, and R.A. Cerione. Insulin-like growth factor-I diminishes the activation status and expression of the small GTPase Cdc42 in articular chondrocytes. J. Orthoped. Res. 22: 436-445 (2004).

R.A. Cerione. Cdc42: New roads to travel. Trends in Cell Biology 14: 127-132 (2004).